| Place | Performance | Athlete | Team |
| 1 | 25.16 | Q: VAN DER MERWE - ZANT | AFFIES MEISIES |
| 2 | 25.35 | Q: ROCHA - SELENA CHRISTINA RODRIQUE | TUKS SPORT |
| 3 | 25.74 | Q: BOTES - ZENIQUE | GARSFONTEIN |
| 4 | 26.01 | -: MAKASI - LOLWETHU | JS |
| 5 | 27.86 | -: OPPERMAN - NADIA | WATERKLOOF |
| 6 | -: MOLOTO - MPHO MOLOTO | JS |