| Place | Performance | Athlete | Team |
| 1 | 24.61 | Q: LEFIFI - WANITA | TUT |
| 2 | 24.99 | Q: COMPAAN - MILA | TUKS |
| 3 | 25.45 | Q: RIEKERT - DELANEY | MENLOPARK |
| 4 | 26.31 | -: BANUTELO - SOPHIE MANGILO | TUKS |
| 5 | 27.31 | -: TSHIKOTA - TSHOLOFELO | TUKS SPORT |
| 6 | 28.42 | -: NATHAN - NICOLE | WILLEBEER |