| Place | Performance | Athlete | Team |
| 1 | 24.80 | Q: COLLINS - YAVE | TUKS |
| 2 | 25.15 | Q: LACY - CASSIDY | TUKS |
| 3 | 25.20 | Q: NOTHNAGEL - LINAMARIE | MIDSTREAM COLLEGE |
| 4 | 25.61 | -: MABUZA - ANDILE | FWAAC |
| 5 | 26.77 | -: RAMAFOKO - MPHO PRECIOUS | TUKS SPORT |
| 6 | 28.83 | -: MAKHUBELA - THANDOLWETHU | JS (SOUTH) |