| Place | Performance | Athlete | Team |
| 1 | 23.52 | Q: NYAKALO - NHLANHLA | TEMBA PRESTIGE COLLEGE |
| 2 | 23.57 | Q: BUTHELEZI - NKULULEKO | JS |
| 3 | 24.09 | Q: MAGUBANE - PHILANI | WILLEBEER |
| 4 | 24.48 | -: MPOPO - LIMPHO BRANDON | TUKS SPORT |
| 5 | 28.39 | -: SHAVA - SAMILA | PHOBIANS |
| 6 | -: CHAUKE - MORATWA | GERRIT MARTIZ |