| Place | Performance | Athlete | Team |
| 1 | 24.55 | Q: NEL - ARIANNE | UFS A |
| 2 | 24.73 | Q: SEKESE - MPONTSHENG | TUT A |
| 3 | 25.07 | q: KLERK - KAYLIN | UWC A |
| 4 | 25.89 | -: JOHNSON - LARA | STELLENBOSCH |
| 5 | 26.35 | -: SANDER - CARIEN | UFS A |
| 6 | 26.44 | -: WAIT - SASKIA | MADIBAZ UNIV |
| 7 | 26.61 | -: HILL - LAWON | UWC A |
| 8 | 26.97 | -: GROENEWALD - TAMZYN | UWC A |