| Place | Performance | Athlete | Team |
| 1 | 23.46 | Q: PALFRAMAN - JUSTINE | STELLENBOSCH |
| 2 | 23.82 | Q: RAUTENBACH - ANE | NWU EAGLES A |
| 3 | 25.09 | q: STRIJDOM - ANGELIQUE | TUT A |
| 4 | 25.69 | -: BYMAN - LAURE | UWC A |
| 5 | 25.75 | -: SAMUELS - CHELSEA | UWC A |
| 6 | 26.25 | -: ALECIA - KEYS | UWC A |
| 7 | 26.64 | -: STOLZ - JANNE | UJ A |
| 8 | 27.87 | -: RHODE - CHELSEA | UWC A |