| Place | Performance | Athlete | Team |
| 1 | 24.56 | Q: VILJOEN - MARLIE | TUKS A |
| 2 | 24.97 | Q: SEKESE - MPONTSHENG | TUT A |
| 3 | 25.86 | Q: SAMUELS - CHELSEA | UWC A |
| 4 | 26.03 | Q: BYMAN - LAURE | UWC A |
| 5 | 26.05 | q: KEMBO - RAISSA | UJ A |
| 6 | 26.85 | -: DU PLESSIS - ELSABE | UFS A |
| 7 | 33.55 | -: NHLAPO - BUSISIWE | CENTRAL UNIV OF TECH |