| Place | Performance | Athlete | Team |
| 1 | 0:46.59 | Q: NENE - ZAK | NWU EAGLES A |
| 2 | 0:46.89 | Q: MATSOSO - ISADORE TSEBO | UFS A |
| 3 | 0:47.09 | Q: ISAACS - GARDEO | STELLENBOSCH |
| 4 | 0:48.04 | q: JOOD - ASHWIN BERNARD | TUKS A |
| 5 | 0:49.37 | -: MATLATA - LESEDI | UJ B |
| 6 | 0:50.40 | -: ISAACS - JERMAINE | STELLENBOSCH |
| 7 | 0:54.16 | -: DURANDT - ANDRE | UJ B |