| Place | Performance | Athlete | Team |
| 1 | 0:46.45 | Q: LOTTERING - DARYLL MASON | MADIBAZ UNIV |
| 2 | 0:46.76 | Q: CONRADIE - PIETER | NWU EAGLES A |
| 3 | 0:47.54 | Q: SETHLABI - ORATILE MCDONALD | UFS A |
| 4 | 0:47.89 | q: OLIPHANT - UNATHI | UFS A |
| 5 | 0:48.21 | -: NKUNKUMANA - ABONGILE | UJ A |
| 6 | 0:49.37 | -: MARICH - NIKOLAS | UJ A |
| 7 | 0:51.13 | -: DAMPIES - GRANT | STELLENBOSCH |