| Place | Performance | Athlete | Team |
| 1 | 0:47.31 | Q: MATSOSO - ISADORE TSEBO | UFS A |
| 2 | 0:47.63 | Q: ISAACS - GARDEO | STELLENBOSCH |
| 3 | 0:48.34 | Q: NKUNKUMANA - ABONGILE | UJ A |
| 4 | 0:51.86 | q: DURANDT - ANDRE | UJ B |
| 5 | 0:53.20 | -: NDLOVU - LUNGISANI | MANGOSUTHU UNIV OF TECH |
| 6 | 0:53.53 | -: VAN ROOYEN - MITCHELL | UWC A |
| 7 | 0:54.03 | -: MODIBA - THOMAS | VUT A |