| Place | Performance | Athlete | Team |
| 1 | 24.72 | Q: MOLEPO - PRECIOUS | TEMBA PRESTIGE COLLEGE |
| 2 | 25.37 | Q: MURRAY - KAYLA | WATERKLOOF |
| 3 | 25.59 | Q: RIEKERT - DELANEY | MENLOPARK |
| 4 | 25.69 | -: WILLIAMS - ASHLEY | MIDSTREAM COLLEGE |
| 5 | 26.79 | -: MAPAPANYANE - OREFEMETSE | TUKS |
| 6 | 29.71 | -: BONGA - GRACE | TUKS |