| Place | Performance | Athlete | Team |
| 1 | 21.51 | Q: LEOTLELA - TLOTLISO GIFT | TUKS A |
| 2 | 22.00 | Q: MASEKO - NHLANHLA | UJ B |
| 3 | 22.43 | Q: CLOETE - KEENAN ARGENAN | UFS A |
| 4 | 22.82 | -: LEKGAU - JONAS | LIMPOPO UNIV |
| 5 | 23.49 | -: MAKUTA - RUPI | UCT |
| 6 | 23.62 | -: METHEMBE - NGQOLA | UCT |
| 7 | 23.90 | -: XHALA - LUNDI | FORT HARE UNIV |