| Place | Performance | Athlete | Team |
| 1 | 2:20.74 | Q: SWIEGERS - CARINA | TUKS A |
| 2 | 2:21.17 | Q: SENOSI - KELETSO | TUT A |
| 3 | 2:21.55 | Q: WIJTENBURG - SANDRA | UJ A |
| 4 | 2:22.11 | q: MULLER - NIENE | TUKS A |
| 5 | 2:22.30 | q: MARAIS - HIELDE | UFS A |
| 6 | 2:23.39 | q: RAMELA - LEBOGANG | UJ A |
| 7 | 2:28.34 | -: PONI - SINESIPHO | FORT HARE UNIV |
| 8 | 2:33.27 | -: ROBINSON - ASSNIQUE | UWC A |