| Place | Performance | Athlete | Team |
| 1 | 0:54.35 | Q: PALFRAMAN - JUSTINE | STELLENBOSCH |
| 2 | 0:55.77 | Q: STRIJDOM - ANGELIQUE | TUT A |
| 3 | 0:57.13 | Q: PIETERSE - LENTE | NWU EAGLES A |
| 4 | 0:57.63 | q: BALOYI - FANISA MELIAH | UFS A |
| 5 | 0:58.61 | -: FOURIE - ANNAME | UFS A |
| 6 | 0:58.85 | -: VAN BERS - CINDY MIEKE | TUKS B |
| 7 | 1:00.46 | -: DU PLESSIS - ELSABE | UFS A |
| 8 | 1:32.21 | -: MABALENG - FAITH | CENTRAL UNIV OF TECH |