| Place | Performance | Athlete | Team |
| 1 | 0:55.70 | Q: VILJOEN - MARLIE | TUKS A |
| 2 | 0:56.96 | Q: HATTINGH - CARLA | NWU EAGLES A |
| 3 | 0:57.22 | Q: SANDER - CARIEN | UFS A |
| 4 | 0:57.23 | q: GIBBON - NICOLA | NWU EAGLES A |
| 5 | 0:58.48 | -: KHOZA - NONDUMISO | TUT A |
| 6 | 1:01.60 | -: VAN HEERDEN - COURTNEY | UWC A |
| 7 | 1:15.77 | -: MNGOMEZULU - THEMBEKILE | CENTRAL UNIV OF TECH |