| Place | Performance | Athlete | Team |
| 1 | 0:48.33 | Q: NENE - ZAK | NWU EAGLES A |
| 2 | 0:48.44 | Q: JOOD - ASHWIN BERNARD | TUKS A |
| 3 | 0:48.82 | Q: MATLATA - LESEDI | UJ B |
| 4 | 0:49.08 | q: NDLOVU - RODWELL | UWC A |
| 5 | 0:50.43 | q: DAMPIES - GRANT | STELLENBOSCH |
| 6 | 0:52.00 | -: NYALUNGA - DENNIS | MPUMALANGA UNIV |
| 7 | 0:53.00 | -: LANGA - MACLAAS | TUT A |
| 8 | 0:54.43 | -: MOSAMEDI - MPHO | VUT A |