| Place | Performance | Athlete | Team |
| 1 | 24.08 | Q: TSHEMESE - BOIPELO | ATHLETICS GAUTENG NORTH |
| 2 | 24.23 | Q: JANSE VAN RENSBURG - SHERE | ATHLETICS CENTRAL NORTHWEST |
| 3 | 24.56 | -: MONGWE - NIKIWE | CENTRAL GAUTENG ATHLETICS |
| 4 | 24.69 | -: SEKESE - MPONTSHENG | ATHLETICS GAUTENG NORTH |
| 5 | 25.19 | -: JOUBERT - IZANEL | ATHLETICS CENTRAL NORTHWEST |
| 6 | 26.26 | -: MOSIME - CAROLINE | ATHLETICS GAUTENG NORTH |
| 7 | 27.26 | -: RAMOS - TAMSYN | CENTRAL GAUTENG ATHLETICS |