| Place | Performance | Athlete | Team |
| 1 | 1:02.07 | Q: LOOTS - ZAN | MENLOPARK |
| 2 | 1:03.23 | Q: MOTLOHI - REFILOE | TEMBA PRESTIGE COLLEGE |
| 3 | 1:03.55 | Q: VAN STADEN - AMY | MENLOPARK |
| 4 | 1:04.84 | -: BOONZAAIER - ZAN | MIDSTREAM COLLEGE |
| 5 | 1:07.27 | -: VAN DER WESTHUIZEN - ELZAAN | MIDSTREAM COLLEGE |