| Place | Performance | Athlete | Team |
| 1 | 22.20 | Q: TYANTINI - TSHEPO | TUKS |
| 2 | 22.33 | q: MATJILA - LERATO | TEMBA PRESTIGE COLLEGE |
| 3 | 22.56 | q: MCOSANA - SIBUSISO | TUKS |
| 4 | 22.92 | -: SHABANGU - BOKAMOSO | TUKS |
| 5 | 23.01 | -: VD WESTHUIZEN - KARL | AFFIES SEUNS |
| 6 | 23.37 | -: MAGOLEGO - THAPELO | FWAAC |
| 7 | 23.42 | -: MQOKOLO - AVIWE | TEMBA PRESTIGE COLLEGE |