| Place | Performance | Athlete | Team |
| 1 | 24.83 | Q: JINGQI - VIWE | TUKS |
| 2 | 24.92 | Q: KOK - CHANE | TUKS |
| 3 | 24.93 | Q: LACY - CASSIDY | WATERKLOOF |
| 4 | 25.19 | q: VAN ROOYEN - HEIKE | MENLOPARK |
| 5 | 25.64 | q: VAN HEERDEN - DEONNE | ZWARKTOP |
| 6 | 26.56 | -: NGHALA - NDZALAMA | TUKS |
| 7 | 27.88 | -: VAN DER MERWE - ZOë | MENLOPARK |
| 8 | 28.35 | -: TSHIKOTA - TSHOLOFELO | TUKS |