| Place | Performance | Athlete | Team |
| 1 | 25.28 | Q: MATSHITSE - REAOBAKA | TUKS |
| 2 | 25.52 | Q: VAN DEN BERG - MENIKE | MENLOPARK |
| 3 | 26.35 | q: NIEMANDT - KARA | MENLOPARK |
| 4 | 27.04 | -: MGQIBELA - BUHLE | TUKS |
| 5 | 27.21 | -: GROBLER - SHARLAS | ZWARKTOP |
| 6 | 27.90 | -: VAN DYK - CARLI | WATERKLOOF |