| Place | Performance | Athlete | Team |
| 1 | 22.26 | Q: NKOANA - Bradley Botshelo | TUKS |
| 2 | 22.57 | Q: TYANTINI - Tshepo Jason | TUKS |
| 3 | 22.99 | q: AVIWE - Mqokolo | PRESTIGE COLLEGE |
| 4 | 23.27 | -: MOGASWE - Keabetswe | UITSIG HIGH |
| 5 | 27.17 | -: MABOTE T63 - Puseletso | ISA ABILITY SPORT CLUB |