| Place | Performance | Athlete | Team |
| 1 | 0:50.51 | q: MEINTJIES - BENU | PUMA INVITATIONAL |
| 2 | 0:51.32 | q: MATOLLA - CLAUDIO | WORCESTER GYMNASIUM |
| 3 | 0:52.01 | q: BRUMMER - NTANDO | MILNERTON HIGH IND |
| 4 | 0:52.34 | q: MEYER - BRAHAM | PAREL VALLEI |
| 5 | 0:53.48 | q: FRANCIES - JAYDEN | WESTCLIFF HIGH SCHOOL |
| 6 | 0:54.44 | -: JONES - ZITRON | VELOCITY |
| 7 | 0:59.31 | -: MATONA - THATO | PUMA INVITATIONAL |