| Place | Performance | Athlete | Team |
| 1 | 0:46.76 | Q: HLUNGWANI - ASHLEY | MASAI AC |
| 2 | 0:46.89 | Q: BARNES - Andrew | TUKS |
| 3 | 0:48.28 | Q: NCAMANE - Jabolane | EC ACTIVE |
| 4 | 0:49.77 | -: MOGAWANE - Kefiloe | MONTANA HS |
| 5 | 0:51.97 | -: WIID - Fourie | WILLEBEER |
| 6 | 0:52.40 | -: DE VILLIERS - Derrick | TUKS |