| Place | Performance | Athlete | Team |
| 1 | 0:49.42 | Q: NKAHLE - Rappier | TEMBA PRESTIGE COLLEGE |
| 2 | 0:50.53 | Q: NORTJIE - Antonie | TUKS |
| 3 | 0:51.93 | Q: REHLAMFU - Musa | MAMELODI AC |
| 4 | 0:53.65 | -: VAN BILJON - Baren | MOOT |
| 5 | 0:53.74 | -: MLINDAZO - Aphiwe | TUKS |
| 6 | 0:55.04 | -: NEL - Marthinus | WOODHILL COLLEGE |