| Place | Performance | Athlete | Team |
| 1 | 0:48.34 | Q: MAHLANGU - Ntando | MASAI AC |
| 2 | 0:49.57 | Q: MADONSELA - Karabo | TUKS |
| 3 | 0:51.15 | Q: MQCINA - Bongani | TUKS |
| 4 | 0:52.44 | -: GREEF - Ruben | MOOT |
| 5 | 0:53.25 | -: DAMOS - Kutlwane | AKASIA |
| 6 | 0:56.28 | -: RATLHAGANA - Alexander | ROSINA SEDIBANE |