| Place | Performance | Athlete | Team |
| 1 | 25.37 | q: KLAAS - Z | PUMA INVITATIONAL |
| 2 | 25.71 | q: VAN SCHALKWYK - JANKE | PIKETBERG |
| 3 | 25.76 | q: VAN DER HEEVER - DESMORIENE | PNA |
| 4 | 26.81 | q: WILSON - EMILY | FAIRMONT |
| 5 | 27.64 | q: FISHER - HILDEGARD | KYLEMORE |
| 6 | 30.20 | -: VENTER - TARA | STRAND HS |