| Place | Performance | Athlete | Team |
| 1 | 25.95 | q: WAIT - SASKIA | PUMA INVITATIONAL |
| 2 | 26.29 | q: ROSSOUW - LYNN | BLOEMHOF |
| 3 | 27.56 | q: KEWANA - MIRENDA | VUSISIZWE |
| 4 | 28.16 | -: LUDICK - GABRIELLA | RHENISH GIRLS HIGH |
| 5 | 34.26 | -: LEUKES - KEZIAH | WP DEVELOPMENT |
| 6 | 34.78 | -: PAULSE - PAULICIA | PUMA INVITATIONAL |