| Place | Performance | Athlete | Team |
| 1 | 0:46.35 | Q: MOGAUANE - OFENTSE | TUKS |
| 2 | 0:46.53 | Q: MAVUNDLA - OSCAR | TUT |
| 3 | 0:48.20 | Q: PENNELLS - QUINTIN | ECAC |
| 4 | 0:48.44 | -: LUBBE - CHRISTIAN | TUKS |
| 5 | 0:48.95 | q: DE VOS - PHILLIP WOUTER | TUKS |
| 6 | 0:49.39 | -: SEPOTOKELE - LETLHOGONOLO | TUT |
| 7 | 0:49.66 | -: VILJOEN - JANSON | TUKS |